Berlin 2015 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 13: Posters: Imaging and Superresolution Optical Microscopy

BP 13.1: Poster

Montag, 16. März 2015, 17:30–19:30, Poster A

Detecting rare Events for sure: Stitched Field-of-view Imaging on the Intelligent Programmable Array Microscope (iPAM) — •Stephan Kramer, Anthony de Vries, Nathan Cook, Donna Arndt-Jovin, and Thomas Jovin — Labor f. Zelluläre Dynamik, Max-Planck-Institut f. biophysikalische Chemie, Am Faßberg 11, 37077 Göttingen

Imaging rare events by confocal microscopy in populations of live cells requires a macroscopic field of view (FOV) of several millimeters in diameter. Standard laser scanning confocal microscopes or spinning disk systems are too slow to capture FOVs that large. Using the scripting capabilities of the driving software of our iPAM [1,2] we are able to seamlessly image regions of the size of 1 mm². The total FOV is subdivided into regular array of tiles corresponding to the size of the FOV of the microscope which depends on the number of pixels in the camera. Usually, the array is of the size of 30 × 30 where at each position a z stack of 10 to 30 images is recorded within a couple of seconds. The scripting facility of the iPAM allows us to analyze the recorded images concurrent to the acquisition so that after a first scan of the total area only those tiles are retained where cells of interest have been detected. As sample application we discuss the recording of UV-triggered protein transport from the endoplasmatic reticulum to the Golgi apparatus.

[1] W. Caarls et al., Minimizing light exposure with the programmable array microscope, J. Microscopy 241, 101 – 110 (2010)

[2] P. De Beule et al. Generation-3 programmable array microscope with digital micro-mirror device. SPIE proceedings (2011)