Berlin 2015 – scientific programme
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BP: Fachverband Biologische Physik
BP 13: Posters: Imaging and Superresolution Optical Microscopy
BP 13.14: Poster
Monday, March 16, 2015, 17:30–19:30, Poster A
SIM microscopy to investigate lipofuscine granules in retinal pigment epithelial cells — •Florian Schock1,2,3,4, Gerrit Best1,2,4, Nil Celik2, Alena Bakulina5,9, Saadettin Sel2, Udo Birk1,3, Rainer Heintzmann6,7,10, Jürgen Hesser5,9, Stefan Dithmar2,8, and Christoph Cremer1,3,4 — 1Kirchhoff Institute for Physics, University of Heidelberg — 2Department of Ophthalmology, University-Hospital Heidelberg — 3Institute of Molecular Biology, University of Mainz — 4Institute of Pharmacy and Molecular Biotechnology, University of Heidelberg — 5Experimenatal Radiation Oncology, University Medical Center Mannheim, University of Heidelberg — 6Institute for Physical Chemistry and Abbe Center of Photonics, University of Jena — 7Leibniz Institute of Photonic Technology — 8Department of Ophthalmology, Hospital Wiesbaden — 9Institute for Scientific Computation, University of Heidelberg — 10Randall Division of Cell & Molecular Biophysics, King's College London
Age related macular degeneration, the main cause for legal blindness in industrial countries, is accompanied by accumulation of lipofuscine granules inside the retinal pigment epithelial cells (RPE cells). Here we demonstrate that the super-resolution technique "Structured Illumination Microscopy" (SIM) is able to resolve up to over 100 granules inside single cells and compare these results with other microscopy techniques. In addition, we introduce an algorithm to automatically identify, separate and characterise the granules, and present first super-resolution images on spectral discrimination of lipofuscine granules and intra-granule regions.