Berlin 2015 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 13: Posters: Imaging and Superresolution Optical Microscopy

BP 13.16: Poster

Montag, 16. März 2015, 17:30–19:30, Poster A

Raman spectroscopic analysis of drug-target interactions in malaria disease — •Torsten Frosch^{1, 2} and Juergen Popp^{1, 2} — ¹Leibniz Institute of Photonic Technology — ²Institute of Physical Chemistry, Friedrich Schiller University Jena

Raman spectroscopy provides extremely high chemical selectivity for the identification and quantification of antimalarial active agents [1,2]. A Raman spectroscopic elucidation of drug-target-interactions was be performed in physiological environment [3], while water did not cause a strong Raman signal. Raman spectra were acquired within intact cells. Highly spectrally resolved Raman difference spectra were acquired in order to elucidate small shifts of the molecular vibrations caused by the weak interactions. Defined stoichiometric ratios of heme (targets) and antimalarial drugs characterize the molecular interaction. With help of a thorough interpretation of these binding processes this work contributes towards the tailored design of new effective active agents against Malaria.

Acknowledgment: We thank Katja Becker for help with cell preparation.

References: [1] Frosch, T.; Yan, D.; Popp, J. Anal Chem 2013, 85, 6264-6271. [2] Frosch, T.; Schmitt, M.; Noll, T.; Bringmann, G.; Schenzel, K.; Popp, J. Anal. Chem. 2007, 79, 986-993. [3] Frosch, T.; Meyer, T.; Schmitt, M.; Popp, J. Anal. Chem. 2007, 79, 6159-6166.