Berlin 2015 – scientific programme
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BP: Fachverband Biologische Physik
BP 13: Posters: Imaging and Superresolution Optical Microscopy
BP 13.9: Poster
Monday, March 16, 2015, 17:30–19:30, Poster A
All-optical realization of optical diffraction tomography — •Mirjam Schürmann1, Paul Müller1, Moritz Kreysing2, and Jochen Guck1 — 1Biotechnology Center, TU-Dresden, Tatzberg 47/49, 01307 Dresden, Germany — 2Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108, 01307 Dresden, Germany
Quantitative phase microscopy is an established marker-free imaging technique for biological cells. Several studies have demonstrated the refractive index (RI) to be a conclusive measure for cellular alterations e.g. due to infection or during differentiation. A 2D mean RI map of a cell can be determined from a phase map if the cell shape is known. However, imaging cells from only one direction leads to a mixed contribution of all cellular components to the measured phase. This prevents an assignment of an RI to individual cellular organelles. Rotating the sample for tomographic imaging can overcome this problem. Here, we present the experimental realization of a contact-free rotation of individual suspended cells in combination with digital holographic microscopy (DHM) for quantitative phase imaging at each of the rotational positions. The rotation is implemented in a dual-beam laser trap. A spatial light modulator (SLM) is used to control the orientation of the LP11 output fiber mode of one of the trapping fibers which leads to a subsequent controlled orientation of the cell. The gathered data can be combined to a 3D RI map of the cell using optical diffraction tomography (ODT). This all-optical demonstration of ODT opens the door to many applications in basic biology and biotechnology.