Berlin 2015 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 14: Posters: Neurophysics
BP 14.8: Poster
Montag, 16. März 2015, 17:30–19:30, Poster A
Computer generated holography for optogenetic modulation of neural network activity in vitro — •Manuel Schottdorf1,2, Hecke Schrobsdorff1, Walter Stühmer2 und Fred Wolf1 — 1MPI für Dynamik und Selbst-Organisation, Göttingen — 2MPI für Experimentelle Medizin, Göttingen
A randomly plated culture of neurons resembles to some extend in-vivo neural tissue in structural features, activity and development [Chiappalone et al. 2006, Huettner & Baughman 1986, Cohen et al. 2008] and has been used for various studies of learning, memory, plasticity, connectivity, and information processing. The activity of neurons in a culture can be measured with multi-electrode arrays. However, providing the cell culture with precise and spatially complex input patters is limited, most obviously by current diffusion from the electrodes.
Here, we address this problem using holography [Golan et al. 2009]. A laser and a spatial light modulator, assembled in the beam path of an inverted microscope, are used to generate holographic interference patterns in the object plane. These precise and spatially complex patterns excite a cell culture of optogenetically modified cortical neurons of a rat. The neural responses are monitored with a multielectrode array.
We present a performance assessment of our setup. We show that cultured neurons react well to external stimuli and we present some preliminary results on the modulations in network activity by the spatially complex optical excitation.