Berlin 2015 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 16: Posters: Cell adhesion, mechanics and migration
BP 16.23: Poster
Montag, 16. März 2015, 17:30–19:30, Poster A
Alteration of rolling adhesion in aged monocytes. — •Samira Khalaji1, Lisa Zondler2, Jochen Weishaupt2, Veselin Grozdanove2, Karin Danzer2, Ulla Nolte1, and Kay-E Gottschalk1 — 1Institut für Experimentelle Physik, Universität Ulm, Ulm, Germany — 2Klinik für Neurologie , Universitätsklinikum Ulm, Ulm, Germany
Aging is associated with a deterioration in immune function. Consequently susceptibility to inflammation and degenerative age-related diseases are increased. This complicated multi-level process is among other factors mediated by activated cells of the innate immune system, such as monocytes. For instance, adhesion of monocytes to the artery walls is an important early step in the development of atherosclerotic lesions. The adhesion of monocytes often takes place at positions with exposed collagen. To study the effect of age on monocyte rolling and adhesion, 'human aged monocyte' (isolated from 8 individuals >48 years old) were compared to 'human young monocytes' (Isolated from 8 individuals 25-36 year old). We measured the adhesion rate and rolling velocity of monocytes on collagen coated microfluidic flow chambers at a shear stress of 0.6 dynes/cm2. The function of cells were compared additionally by their ability to be activated by lipopolysaccharide. Our results shows a significantly higher number of firmly adhered aged monocyte compared to the young monocytes (P=0.022) in LPS stimulated monocytes. This study shows that aging is associated with alterations in monocytes function, which may have beneficial implications for the development of studies regarding age-related diseases.