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BP: Fachverband Biologische Physik

BP 16: Posters: Cell adhesion, mechanics and migration

BP 16.9: Poster

Montag, 16. März 2015, 17:30–19:30, Poster A

Investigation of Cell Adhesion and Motility on Microstructured Substrates — •Daniel Geiger¹, Ulla Nolte¹, Susanne Rappl¹, Michael Beil², and Othmar Marti¹ — ¹Institute of Experimental Physics, Ulm University — ²Department of Internal Medicine, University Hospital Ulm

Interaction between cells and artificial materials is of prime importance for many medical applications like implant technology. An interface can be considered as external stimulus and therefore, for example, affecting differentiation and viability of cells.

For that reason, the behaviour of cells on microstructured substrates is investigated by means of fluorescence microscopy, electron microscopy and common video microscopy. Main emphasis is put on the study of their adhesion properties, e.g. the distribution and formation of focal adhesions. Therefore, immunostaining of specific proteins like vinculin and surface sensitive techniques like total internal reflection microscopy (TIRFM) or electron microscopy of surface sections are used.

Structuring of the substrates is done by UV exposure of a PLL-g-PEG layer on glass through a mask that enables creation of features as small as one micrometer. Subsequent addition of fibronectin creates a strong contrast to the non-illuminated PEG covered sites.