Berlin 2015 – scientific programme

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BP: Fachverband Biologische Physik

BP 17: Posters: Protein structure and dynamics

BP 17.21: Poster

Monday, March 16, 2015, 17:30–19:30, Poster A

Single-molecule stochastic modeling of the channeling enzyme tryptophan synthase — •Dimitri Loutchko and Alexander S. Mikhailov — Fritz Haber Institute of the Max Planck Society

The channeling enzyme tryptophan synthase provides a paradigmatic example of a chemical nanomachine. It possesses two active centers and, as a single molecule, catalyzes a sequence of 13 different reactions with a complex pattern of allosteric regulation and with an intermediate product channeled from one active center to another. Here, the first single-molecule stochastic model of the enzyme is proposed and analyzed. All its transition rate constants were deduced from the experimental data available and no fitting parameters were thus employed. Numerical simulations reveal the development of strong correlations in the states of the active centers and the emergent synchronization of intramolecular processes in tryptophan synthase. While performed for a specific enzyme, this study sets a framework for stochastic modeling of other chemical machines, such as channeling enzymes and multi-enzyme complexes.