Berlin 2015 – scientific programme

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BP: Fachverband Biologische Physik

BP 17: Posters: Protein structure and dynamics

BP 17.6: Poster

Monday, March 16, 2015, 17:30–19:30, Poster A

Insoluble proteins in presence of salt: a computational study — •Patrick Kreissl and Jens Smiatek — Institut für Computerphysik, Universität Stuttgart, 70569 Stuttgart, Germany

Nogo-60 is a truncated sixty residue version of the extracellular domain of the human Nogo proteins. It is soluble in pure water but highly insoluble in buffer. Surprisingly, the protein almost completely consists of three large α-helices. However, the last six residues remain highly unstructured. If this six residue tail is dropped, another protein—Nogo-54—is designed, which is soluble in both buffer and pure water. Nogo-60 and Nogo-54 thus provide an almost identical primary structure but different solubility/insolubility properties.

Both proteins were simulated in different salt solutions as well as in pure water to get an idea of the underlying mechanisms and to study the influence of the salt ions on the protein solvation behavior.