Berlin 2015 – scientific programme

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BP: Fachverband Biologische Physik

BP 18: Membranes and vesicles I (joint BP/CPP)

BP 18.10: Talk

Tuesday, March 17, 2015, 12:15–12:30, H 1028

Fluorescence Imaging of Light Induced Reactive Oxygen Species (ROS) in Plant Cell Tissue — •Franz-Josef Schmitt¹, Vladimir Kreslavski², Galina N. Schirshikova², Csongor Keuer¹, Sergei K. Zharmukhamedov², Suleyman I. Allakhverdiev², and Thomas Friedrich¹ — ¹Institute of Chemistry, Bioenergetics, TU Berlin, Berlin, Germany — ²Institute of Basic Biological Problems, RAS, Pushchino, Moscow Region, Russia

UV-radiation in combination with toxic compounds like polyaromatic hydrocarbons (PAHs) lead to generation and accumulation of reactive oxygen species (ROS) in animal and plant cells. ROS generation by naphthalene (Naph), a lipophilic PAH, was studied with fluorescence microscopy employing the ROS sensitive dye dichlorofluorescein (DCF). Under high light illumination, Naph-treated leaves of Arabidopsis thaliana showed the spread of ROS waves across the tissue with a period time of 20 min. The reduction of PSII activity at the presence of Naph was accompanied by transient generation of hydrogen peroxide as well as swelling of thylakoids and distortion of cell plasma membranes. It could be shown that Naph treated leaves of Arabidopsis thaliana show enhanced DCF fluorescence in the thylakoid membrane. The comparison of short term and long term exposure to different PAHs revealed that at short term exposure, the PAHs with high water solubility lead to the strongest reduction of PS II activity while after long term exposure the effect of PAHs with low water solubility is stronger.