Berlin 2015 – scientific programme
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BP: Fachverband Biologische Physik
BP 22: Posters: Cytoskeletal filaments
BP 22.5: Poster
Tuesday, March 17, 2015, 14:00–16:00, Poster A
Molecular assembly studied in microfluidic channels using fluorescence cross correlation spectroscopy — •Viktor Schroeder1, Bernd Nöding1, Harald Herrmann2, and Sarah Köster1 — 1Institute for X-Ray Physics, Georg-August-Universität Göttingen, Germany — 2Division of Molecular Genetics, German Cancer Research Center (DKFZ), Heidelberg, Germany
We present a combination of microfluidic diffusive mixing and single wavelength fluorescence cross correlation spectroscopy (SW-FCCS) to study rapid molecular assembly processes. In SW-FCCS, information about diffusing fluorescent particles is retrieved by analyzing the (cross-)correlation of intensity fluctuations. One laser line is used to excite two different fluorescent dyes with separated emission spectra. To overcome the limited temporal resolution of SW-FCCS, we use continuous flow microfluidic tools to map the temporal evolution to a spatial axis. The macromolecules of interest flow down a channel and data are collected at different positions along the channel. To get a narrow distribution of first contact times, the central jet is hydrodynamically focused to a thin sheet. Molecular assembly processes are initiated by the diffusion of trigger molecules into the central stream of macromolecules. As an example, we employ this method for studying the assembly of intermediate filament proteins like vimentin.