Berlin 2015 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 22: Posters: Cytoskeletal filaments

BP 22.6: Poster

Dienstag, 17. März 2015, 14:00–16:00, Poster A

Microrheological Properties of Keratin 8/18 Networks — •Tobias Neckernuss¹, Ines Martin¹, Katinka Mertens¹, Tobias Paust¹, Harald Herrmann², Michael Beil³, and Othmar Marti¹ — ¹Institute for Experimental Physics, Ulm University — ²Division of Molecular Genetics, German Cancer Research Center, Heidelberg — ³Internal Medicine I, Ulm University

The cytoskeleton of epithelial cells consists of three types of filament systems: microtubules, intermediate filaments and actin filaments. In our work, we have a closer look on intermediate filament networks consisting of keratin 8/18 and the crosslinker MgCl₂. With an optical tweezers we are able to determine mechanical properties of the network by trapping and exciting an embedded polystyrene bead to oszillations. The moving bead exerts a force which is transferred via the network to response beads in the surrounding. Correlating the motion of the excited beads with the ones of the response beads allows us to determine the isotropy of the network. In this context we take a deeper look on the conversion of the mean squared displacement (MSD) into the shear modulus and compare the results for active and passive multi-particle microrheology.