Berlin 2015 – scientific programme
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BP: Fachverband Biologische Physik
BP 24: Posters: Membranes and vesicles
BP 24.10: Poster
Tuesday, March 17, 2015, 14:00–16:00, Poster A
Fabrication of Giant Unilamellar Vesicles — •Hannah Stein1,2, Susann Spindler1, and Vahid Sandoghdar1 — 1Max Planck Institute for the Science of Light Günther-Scharowsky-Str. 1 D-91058 Erlangen — 2Friedrich-Alexander-Universität Erlangen-Nürnberg D-91058 Erlangen
Giant Unilamellar Vesicles (GUVs) offer a useful model system for studying fundamental biophysical properties such as lipid diffusion or the interaction of proteins or viruses with membranes. A variety of fabrication methods for GUVs in low ionic solutions already exist, however the formation of giant vesicles under physiological conditions still poses a challenge. We present methods for preparing GUVs in the range of 50-100um diameter in high ionic buffers with different osmolarity using electroformation on ITO covered glasses or lipid swelling on a polymer layer.