Berlin 2015 – scientific programme

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BP: Fachverband Biologische Physik

BP 24: Posters: Membranes and vesicles

BP 24.7: Poster

Tuesday, March 17, 2015, 14:00–16:00, Poster A

Three-dimensional fluorescence-free tracking of a nanoparticle on a giant unilamellar vesicle — •Susann Spindler, Jens Ehrig, and Vahid Sandoghdar — Max-Planck-Institute for the science of light, Erlangen, Germany

Among the diversity of model membrane systems, giant unilamellar vesicles (GUVs) represent a favourable model system for a cell membrane because of their compatible sizes and shape. Furthermore, GUVs provide a convenient platform for studying basic membrane phenomena in a model system without the artefacts of a substrate. We report on using interferometric scattering (iSCAT) microscopy as a fluorescence-free technique to study the three-dimensional diffusion of single particles such as virus particles and gold particles attached to lipids on a GUV. iSCAT is based on measuring the interference of the Rayleigh scattering with a reference light beam and allows for high temporal resolution as well as nanometer precision in particle tracking. Moreover, iSCAT provides high-resolution information on the axial displacement of the particle, allowing one to map the curved GUV surface. We compare our observations of lipid diffusion in GUVs with those in supported lipid bilayers [1].

[1] C.-L. Hsieh, S. Spindler, J. Ehrig, and V. Sandoghdar, J. Phys. Chem. B 118 (2014)