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Berlin 2015 – scientific programme

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BP: Fachverband Biologische Physik

BP 25: Posters: DNA/RNA and related enzymes

BP 25.6: Poster

Tuesday, March 17, 2015, 14:00–16:00, Poster A

Measuring DNA translocation forces through various solid state nanopores with Optical Tweezers — •Sebastian Knust, Andy Sischka, and Dario Anselmetti — Experimental Biophysics & Applied Nanoscience, Faculty of Physics, Bielefeld University, 33615 Bielefeld, Germany

We measured the forces acting on a single strand of dsDNA during translocation through nanopores in various solid state membranes by Optical Tweezers. The system includes a video-based force detection and analysis system allowing for virtually interference-free axial force measurements with sub-piconewton precision [1]. All measurements were performed with an overall force resolution of 0.5 pN at a sample rate of 123 Hz.

We show the controlled translocation through Si2N3 membranes both uncoated and lipid-coated [2]. Additionally, measurements of controlled dsDNA translocation through carbon nanomembranes (CNM) and through MoS2 membranes were conducted.

Furthermore, measuring controlled translocation through graphene nanopores was found challenging, due to local heating phenomena encountered upon approaching an optically trapped microbead to a free standing graphene membrane. We analysed these phenomena in detail.

[1] S. Knust et. al., Rev. Sci. Instrum. 83, 103704 (2012)

[2] L. Galla et. al., Nano Lett. 14, 4176 (2014)

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