Berlin 2015 – scientific programme
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BP: Fachverband Biologische Physik
BP 29: Posters: Systems biology
BP 29.2: Poster
Tuesday, March 17, 2015, 14:00–16:00, Poster A
combined x-ray phase contrast tomography and scanning x-ray micro-diffraction for multi-scale investigation in regenerative medicine — •alessia cedola1, gaetano campi2, inna bukreeva1, michela fratini1, giuliana tromba3, maddalena mastrogiacomo4, ranieri cancedda4, and manfred burghammer5 — 1ipcf-cnrc/o Physics Departement- Sapienza University- Rome-Italy. — 2Istituto di Cristallografia- CNR, Monterotondo Rome, Italy. — 3Sincrotrone Trieste SCpA, 34149 Basovizza (Trieste), Italy. — 4Dipartimento di Medicina Sperimentale-Università di Genova — 5esrf-Grenoble-France
The importance of investigating the engineered bone, which is formed when porous ceramic constructs are loaded with bone marrow stromal cells and implanted in vivo, is a key point for Regenerative Medicine. Several aspects of the mechanisms leading to the generation of the new bone,in particular the dynamics of collagen packing during mineralization, requires a deeper understanding. The hierarchical structure of bone makes the combination of X-ray micro-diffraction scanning technique (XR*D) and the X-ray phase contrast tomography (XRPCT) the most effective tool to investigate the structural features of this tissue at different length scales.In particular we use XRPCT to provide the direct 3D image of the collagen network organization and XR*D to probe the structural fluctuations of the collagen lateral spacing and orientation during the growth of mineral particles at molecular and atomic scale. Moreover, we image by XRPCT the 3D microvascular networks in different bone-engineered constructs.