Berlin 2015 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 33: Cell adhesion, mechanics and migration I (joint BP/CPP)
BP 33.5: Vortrag
Mittwoch, 18. März 2015, 10:45–11:00, H 1058
Molecular stress sensors constructed from DNA — •Meenakshi Prabhune1, Jonathan Bath2, Andrew Turberfield2, Florian Rehfeldt1, and Christoph F Schmidt1 — 1Third Institute of Physics-Biophysics, Georg August University, Göttingen, Germany — 2University of Oxford, Department of Physics, Clarendon Laboratory, Parks Road, Oxford OX1 3PU, UK
Molecular stress generation in cells is spatially and temporarily organized in complex patterns to drive meso-scale active processes such as intracellular transport, cell migration, or cell division. To quantitatively understand how these processes are driven, it is necessary to map local stresses inside cells, which is hard due to the lack of appropriate probes. We have designed a molecular-scale probe consisting of a self-assembled DNA hairpin with a fluorophore - quencher pair that responds to small forces applied to its ends. We demonstrate the working of this force sensor in vitro and explore possibilities for in vivo application to map local stress fields in cells.