Berlin 2015 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 36: Cell adhesion, mechanics and migration II

BP 36.8: Vortrag

Mittwoch, 18. März 2015, 17:15–17:30, H 1058

Characterizing Cell Motility and Transmigration on Ring Shaped Micro Patterns — •Christoph Schreiber, Felix Jakob Segerer, and Joachim Oskar Rädler — Fakultät für Physik, Ludwig-Maximilians-Universität München, Germany

Cell migration is important in many biological processes such as embryogenesis, wound healing, or cancer metastasis. To understand the formation of tumors and the effect of drugs, a detailed characterization of the migration behavior is important. Furthermore the ability to overcome barriers like the basement membrane is a key indicator for the aggressiveness of different cancer cells. Therefore a systematic approach for studying transmigration behavior is necessary to characterize the invasiveness of cancer cells.
Here, we study single cell migration constrained to a micro-patterned ring-shaped lane. On such tracks cells perform a 1D persistent random walk like movement that can be divided in a directional and a reorientation phase. Analyzing large arrays in parallel, we are able to evaluate characteristic velocities and persistence times of a cell line with high accuracy. By introducing a gap of defined size and chemical composition in the ring shaped lane we study how cell migration is affected by the encounter with a chemical barrier. At the chemical border cells either turn around or transmigrate over the barrier. Studying the transmigration probability systematically, we find a steady decrease of transition probability with increasing barrier width.