Berlin 2015 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 41: Protein structure and dynamics I
BP 41.11: Vortrag
Donnerstag, 19. März 2015, 12:45–13:00, H 1058
Hierarchical nanoscale dynamics of proteins in solution — •Marco Grimaldo1,2, Felix Roosen-Runge1, Fajun Zhang2, Frank Schreiber2, and Tilo Seydel1 — 1Institut Laue-Langevin, Grenoble, France — 2Institut für Angewandte Physik - Universität Tübingen, Tübingen, Deutschland
The dynamics of proteins in solution is essential for both protein function and cellular processes. The hierarchical protein dynamics, from the global center-of-mass diffusion to the motions of side-chains and chemical groups renders a complete understanding challenging. Profiting from the very high flux of the new backscattering spectrometer IN16B, the translational and rotational diffusion of the proteins can be self-consistently separated from the internal molecular motions. The global protein diffusion on the nanosecond time scale is consistent with predictions for colloidal suspensions of effective hard spheres even when the molecular structure differs considerably from a sphere [1]. The internal motions on nanometer length scales are characterized both geometrically and dynamically, suggesting a picture of methyl rotations and restricted diffusion of side chains. We also systematically explore the temperature-dependence of both the global and internal diffusive motions in protein solutions, including unfolding and aggregation at temperatures beyond the thermal denaturation [2].
[1] M. Grimaldo, F. Roosen-Runge, F. Zhang, T. Seydel, F. Schreiber, J. Phys. Chem. B 118, 7203 (2014) [2] M. Grimaldo, F. Roosen-Runge, N. Jalarvo, M. Zamponi, F. Zanini, M. Hennig, F. Zhang, F. Schreiber, T. Seydel, submitted