Berlin 2015 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 41: Protein structure and dynamics I
BP 41.8: Vortrag
Donnerstag, 19. März 2015, 12:00–12:15, H 1058
Structural mechanics of 2D and 3D lattices from clathrin proteins — •Mitja Platen1, Philip N. Dannhauser2, Heike Böning2, Huberta Ungewickell2, Ernst Ungewickell2, and Iwan A.T. Schaap1 — 1IIIrd Institute of Physics, Georg August University Göttingen, Germany — 2Institute of Cell Biology, Centre of Anatomy, Hannover Medical School, Hannover, Germany
In the cell clathrin proteins can form polyhedral scaffolds that facilitate the formation of highly curved vesicles (~100 nm) from lipid bilayers. These vesicles are involved in intracellular transport but also in the transport of compounds in and out the cell. We recently developed the methodology to reconstitute clathrin lattices in a minimal system, with and without lipid bilayers. With AFM imaging we are able to reconstruct the orientation of the clathrin triskelia in a planar lattice and to quantify the mechanical role of the clathrin light chains in the lattice. Our current efforts are aimed to measure the forces that are exerted by clathrin lattice to bend the lipid bilayer into vesicles.