Berlin 2015 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 42: Cytoskeletal filaments (joint BP/CPP)
BP 42.10: Vortrag
Donnerstag, 19. März 2015, 12:30–12:45, H 1028
Organisation dynamics of stress fibers in adult stem cells — •Carina Wollnik1, Benjamin Eltzner2, Stephan Huckemann2, and Florian Rehfeldt1 — 1Third Institute of Physics - Biophysics, Georg-August-University, Göttingen, Germany — 2Institute for Mathematical Stochastics, Georg-August-University, Göttingen, Germany
Adult human mesenchymal stem cells (hMSCs) differentiate into various cell types. Here substrate stiffness is sufficient to guide hMSCs towards different lineages without additional biochemical stimuli [1]. Stress fibres (SFs)composed of actin filaments, cross-linkers and myosin motor-proteins generate and transmit tension throughout the cell. Myosin inhibition stops the differentiation [1], implying importance of SF tension for this process. Characteristic SF patterns can be detected within 24 hours and used as an early morphological marker [2].
We use 24h long-term live-cell imaging of RFP-Lifeact transfected hMSCs on substrates of different stiffness, recording many cells in parallel for better statistics in comparable conditions. SFs are traced with a sophisticated filament tracking program [3] and a tool to extract filament modes [4], to gain a deeper understanding of SF formation dynamics in early stem cell differentiation. This leads to a non-monotonic dependence of SF polarization on the Young’s modulus of the underlying substrate[2].
[1] A. Engler et al., Cell (2006); [2] A. Zemel et al., Nature Physics (2010); [3] B. Eltzner et al., arXiv:1408.4002, 2014; [4] S. Huckemann et al., arXiv:1404.3300, 2014;