Berlin 2015 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 46: Biomaterials and Biopolymers II (joint CPP/BP)
BP 46.5: Vortrag
Donnerstag, 19. März 2015, 16:00–16:15, C 264
Characterization of a liposomal drug carrier with continuous contrast variation in SAXS — •Raul Garcia-Diez1, Christian Gollwitzer1, Michael Krumrey1, and Zoltan Varga2 — 1Physikalisch-Technische Bundesanstalt (PTB), Abbestr. 2-12, 10587 Berlin, Germany — 2Biological Nanochemistry Research Group, Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Magyar Tudosok korutja 2, H-1117, Budapest, Hungary
Doxorubicin is an anticancer drug known for its high cardiotoxicity, though a liposomal formulation of it can reduce this side-effect significantly and improve the pharmacokinetics of the drug. In this work, the mean size and average density of pegylated liposomal doxorubicin (Caelyx®) was determined by continuous contrast variation in SAXS with iodixanol, an iso-osmolar suspending medium. The study is focused on the isoscattering point position and the analysis of the Guinier region of the scattering curves recorded at different solvent densities at the four-crystal monochromator beamline of PTB at the synchrotron radiation facility BESSY II. The response of the liposome to increasing solvent osmolality and the structure of the liposome-encapsulated doxorubicin fiber after the osmotic shrinkage of the liposome are evaluated with sucrose contrast variation in SAXS/WAXS.