Berlin 2015 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 53: Complex Fluids and Soft Matter (joint BP/DY/CPP)
BP 53.2: Vortrag
Freitag, 20. März 2015, 09:45–10:00, H 1058
Optical Shaking of Single Cells — •Carla Zensen1,3, Isis E. Fernandez2,3, Oliver Eickelberg2,3, Theobald Lohmüller1,3, and Jochen Feldmann1,3 — 1Chair for Photonics and Optoelectronics, Physics Department and CeNS, Ludwig-Maximilians-Universität, Munich, Germany — 2Comprehensive Pneumology Center, Institute of Lung Biology and Disease, Ludwig-Maximilians-Universität and Helmholtz Zentrum, Munich, Germany — 3Nanosystems Initiative Munich (NIM), Schellingstr. 4, Munich, Germany
We report on a new strategy to dynamically manipulate single cells by exposing them to an applied optical force field which varies periodically in time and space. The mechanical transient response of the cell is monitored both by optical imaging and by a microfluidic detector bead [1] positioned in the cell vicinity. These optical 'shaking' experiments give insight into the mechanobiological properties of single cells.
A predefined array of NIR laser beams is spatially varied with a periodic dynamics in order to optically 'shake' single cells. A detector bead, which is optically trapped with an independent laser beam, is used to simultaneously map the resulting microfluidic flow. We demonstrate a first application of this novel technique by resolving mechanobiological differences in the hypotonic state of individual human erythrocytes. By analyzing the Fourier spectra of cell and detector bead movements, we show that tracking a single detector particle is sufficient to distinguish between soft and hard cells.
[1] A. Ohlinger, A. Deak, A.A. Lutich, and J. Feldmann, Phys. Rev. Lett. 108, 018101 (2012)