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BP: Fachverband Biologische Physik

BP 7: Superresolution Optical Microscopy (focus session)

BP 7.1: Hauptvortrag

Montag, 16. März 2015, 14:30–15:00, H 1028

Super-resolution imaging of small, fast moving cellular structures — •Alexander Rohrbach — Lab for Bio- and Nano-Photonics, University of Freiburg, Georges-Koehler-Allee 102, 79110 Freiburg, Germany

Many new, exciting imaging techniques have emerged during the last decade, providing significantly improved spatial resolution and contrast. However, this extra information comes at the cost of more photons required to illuminate the cell, which requires more time and energy and often damages biological structures. The smaller the structures to be investigated, the faster they usually move inside living cells, because of both Brownian motion and coordinated work of molecular motors. Therefore, alternative imaging approaches have to be developed. In this talk I will demonstrate how fluorescence-based super resolution microscopy uncovers the work of polymerization motors driving the cytoskeleton in bacteria. I will make the switch to ultra- fast, label-free, coherent imaging through scattering of a rotating laser beam, which reveals unexpected biophysical transport processes at the periphery of macrophages. And last, I will show how fast shape changes of a tiny helical bacterium held in a scanning optical trap can be monitored in 3D at 1000 Hz giving insights of molecular processes inside the 200 nm thin cell body.