Berlin 2015 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 7: Superresolution Optical Microscopy (focus session)
BP 7.3: Vortrag
Montag, 16. März 2015, 15:15–15:30, H 1028
Quantitative Analysis of Nuclear Genome Nanostructure using Super-resolution Fluorescence Microscopy — •Christoph Cremer1,2,3, Aleksander Szczurek1, Hyun Keun Lee1,3, Kirti Prakash1,2, and Udo Birk1,3 — 1Institute of Molecular Biology (IMB), D-55128 Mainz/Germany — 2Institute for Pharmacy and Molecular Biotechnology (IPMB), University Heidelberg & Kirchhoff-Institute for Physics (KIP), D-69120 Heidelberg/Germany — 3Department of Physics, University Mainz (JGU), D-55128 Germany
Numerical models of nuclear genome structure have provided quantitative predictions on various length scales, from the micrometer to the nanometer range. Until recently, experimental tests of such models using far field light microscopy were limited by the conventional resolution limit of about 200 nm in the object plane and 600 nm along the optical axis (*Abbe/Rayleigh-limit*). These limits have been overcome by various super-resolution fluorescence microscopy (SRM) methods, such as Stimulated Emission Depletion (STED) and Photoactivated Localization Microscopy (PALM). Here, we report on quantitative nuclear nanostructure analysis based on complementary SRM approaches. Presently, these approaches allow us to analyze nuclear nanostructures down to few tens of nanometer in 3D using a special variant of localization microscopy, Spectral Precision Distance/Position Determination Microscopy (SPDM).