Berlin 2015 – scientific programme
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BP: Fachverband Biologische Physik
BP 7: Superresolution Optical Microscopy (focus session)
BP 7.4: Talk
Monday, March 16, 2015, 15:30–15:45, H 1028
Recent Advances in Pattern Matching based Multi-Species FLIM Analysis — •Felix Koberling1, Benedikt Krämer1, Thomas Niehörster2, Anna Löschberger2, Marcelle König1, Paja Reisch1, Matthias Patting1, Ingo Gregor3, Markus Sauer2, and Rainer Erdmann1 — 1PicoQuant GmbH, Rudower Chaussee 29, 12489 Berlin, Germany, info@picoquant.com — 2Julius-Maximilians-University Wuerzburg, Germany, Department of Biotechnology & Biophysics, Am Hubland, 97074 Wuerzburg, Germany — 3Georg-August-University Göttingen, 3rd Institute of Physics, Friedrich-Hund-Platz 1, 37077 Göttingen, Germany
A pattern matching based fluorescence decay analysis is a successful alternative to multi-exponential decay fitting and to the phasor analysis approach. We describe the complex single pixel decay with a linear combination of reference decays (patterns) and calculate analytically the individual amplitudes for the reference patterns followed by a re-iteration with MLE fitting. This procedure is now further accelerated by a purely analytical vector projection based method. We will compare the different approaches for multi-species FLIM analysis and present latest application examples also combining spectral and lifetime information for a multidimensional fluorescence pattern analysis.