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BP: Fachverband Biologische Physik

BP 7: Superresolution Optical Microscopy (focus session)

BP 7.5: Vortrag

Montag, 16. März 2015, 16:00–16:15, H 1028

Superresolution with Transient Binding: Getting all the Photons — •Philip Tinnefeld — Institut für Physikalische & Theoretische Chemie, TU Braunschweig, Germany

The resolution of localization based superresolution microscopy is intricately connected to the number of photons detected from a single molecule per localization event. We developed DNA PAINT, a superresolution technique with single-molecule switching induced by transient binding of a short labeled oligonucleotide to structures labeled with a complementary nucleic acid strand. Because no photophysical dark-states are involved, the maximum number of photons can be extracted from each fluorescent dye. With DNA PAINT, we resolved two distinct locations (docking strands) at a distance of 6 nm on DNA origami nanostructures. Besides resolution, DNA PAINT offers exquisite multiplexing capabilities without inducing chromatic aberrations. Just by using different sequences, different structures can be imaged. As the number of detectable photons is ultimately connected to the photostability of fluorescent dyes we will also present a new single-protectant mechanism that represents an advancement over the established ROXS-concept for photostabilization and blinking.