Berlin 2015 – wissenschaftliches Programm
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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 34: P6: Biomaterials and Biopolymers
CPP 34.7: Poster
Dienstag, 17. März 2015, 14:00–16:00, Poster C
The adsorption of a cellulose binding module on cellulose surfaces — •Sergio Mauri1, Jim Pfaendtner2, Mischa Bonn1, and Tobias Weidner1 — 1Max Planck institute for polymer research, Mainz, Germany — 2Dept. of chemical engineering, University of Washington, Seattle, US
The conversion of biomass, like other biological processes, is rate-controlled by interfacial phenomena. In the case of enzymatic biomass conversion, cellulose enzymes present specific carbohydrates binding modules (CBM) to provide an increase in concentration of enzyme sites near the cellulose interface. Here we propose to study the interaction of a CBM with cellulose surfaces by a combination of methods, to quantify the adsorption of CBM on cellulose surfaces and to elucidate the CBM/cellulose interfacial structure. We follow the concentration dependent adsorption kinetics of CBM on cellulose surfaces by means of QCM-D and we determine the Langmuir adsorption isotherm. On the same substrate we perform XPS to compare wet and dry adsorbed mass. We finally determine the orientation of the adsorbed protein on such cellulose surfaces by using sum frequency spectroscopy (SFG). SFG is a nonlinear optical technique which provides surface-specific vibrational spectra of sub-monolayers of interfacial molecules. SFG, in conjunction with simulation results, is well-suited for determining the orientation of CBM on cellulose surfaces.