Berlin 2015 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
CPP: Fachverband Chemische Physik und Polymerphysik
CPP 39: Cell adhesion, mechanics and migration I (Joint session BP, CPP)
CPP 39.12: Vortrag
Mittwoch, 18. März 2015, 13:00–13:15, H 1058
PAR polarity pattern in C. elegans zygotes establishes via a mechanochemical feedback module — •Peter Gross1,2, K.Vijay Kumar2,3, Nathan W. Goehring4, Justin S. Bois5, Frank Jülicher3, and Stephan W. Grill1,2,3 — 1MPI-CBG, Dresden — 2BIOTEC, TU Dresden — 3MPI-PKS, Dresden — 4London Research Institute, UK — 5UCLA, Los Angeles, CA
The interplay between biochemistry and cell mechanics is critical for a broad range of morphogenetic changes. A prominent example hereof is the emergence of cell polarity during the embryogenesis of C. elegans, resulting in a patterned state of the membrane-associated PAR polarity proteins. Crucial for the emergence of the patterned state are large-scale flows in the membrane-associated actomyosin cortex, which are observed concomitantly with the emergence of PAR polarization. The coupling of biochemistry and cortical flows, driving this mechanochemical patterning processes, remain poorly understood. Here we establish that PAR polarization of C. elegans zygotes represents a coupled mechanochemical feedback system. We demonstrate that the biochemistry in form of the PAR domains controls mechanics by establishing a myosin gradient. We measure the spatiotemporal profile of the anterior and posterior PAR concentration, the myosin concentration and the induced flow-field. Furthermore we present a theoretical description of this process in the framework of active fluids combined with PAR biochemistry in a coupled reaction-diffusion active-fluids approach. We show that this mechanochemical feedback description quantitatively recapitulates the spatiotemporal profile of PAR polarity emergence.