Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe

CPP: Fachverband Chemische Physik und Polymerphysik

CPP 39: Cell adhesion, mechanics and migration I (Joint session BP, CPP)

CPP 39.9: Vortrag

Mittwoch, 18. März 2015, 12:15–12:30, H 1058

Buckling dynamics of freely diffusing single erythrocytes — •Michael Göllner, Adriana C. Toma, and Thomas Pfohl — Department of Chemistry, University of Basel, Switzerland

Containing a wealth of information, human blood is the most used sample for diagnostic purposes. Microfluidics, with its unique advantages in performing analytical functions, has been increasingly used for whole blood and cell-based analysis. However, studies on the single-cell level using microfluidic techniques often require active immobilization in order to be investigated by optical methods.

We developed a microfluidic setup for single red blood cell (RBC) assays starting with whole blood samples which permits diffusion-controlled variation of the external environment. Individual RBCs are freely diffusing inside microchambers without adhesive interactions to the glass coverslip or the use of optical tweezers. By increasing the surrounding osmotic pressure, erythrocytes are exhibiting a buckling transition which is described by means of radial Fourier analysis. Temporal evolution of the modal decomposition of cell edge movement leads to characterization of osmolarity-dependent fluctuations of freely diffusing single RBCs.