Berlin 2015 – scientific programme
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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 43: P8: Nanoparticles and Compsite Materials
CPP 43.25: Poster
Wednesday, March 18, 2015, 10:00–13:00, Poster A
Specific detection of molecules with NanoSPR — •Sirin Celiksoy1, Rubén Ahijado-Guzmán1, Andreas Henkel1, Janak Prasad1,2, Christina Rosman1, Germán Rivas3, and Carsten Sönnichsen1 — 1Institute of Physikal Chemistry, University of Mainz, Germany — 2Graduate School Materials Science in Mainz, University of Mainz, Germany — 3Centro de Invesitigaciones Biológicas, Madrid, Spain
Nanoparticles hold potential to be used in multiplexed, inexpensive, fast medical diagnostic assays, e.g. for the identification of novel influenza strains. Common approaches for parallel analyte detection in small liquid samples link specific receptor molecules to spectrally encoded markers, thus limiting the number of targets in a parallel assay to a few dozen. Micro-spot arrays where targets bind in pre-defined positions could overcome these limitations. However, current multiplexed detection schemes are too complex, slow and/or expensive for routine use in the point-of-care environment. We show a new approach to detect multiple analytes simultaneously in a microfluidic flow cell using randomly deposited gold nanorods. Each nanorod responds with a spectral shift of its plasmon resonance specifically to one target, acting effectively as a NanoSPR device. Here, we demonstrate the feasibility of the concept, its sensitivity in the nanomolar range, sensor reusability over consecutive cycles, and the potential for up-scaling the concept. Our technique has the potential to simplify multiplexed detection and substantially reduce the costs of production, especially if combined with advanced nanofabrication methods.