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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 46: P11: Wetting, Micro and Nano Fluidics

CPP 46.9: Poster

Mittwoch, 18. März 2015, 10:00–13:00, Poster A

Modelling surface binding and dissociation of biomolecules — •Daniel Kappe^1,2, Andreas Hütten¹, and Christian Schröder² — ¹1Center for Spinelectronic Materials and Devices, Physics Department, Bielefeld University, Germany — ²2Bielefeld Institute for Applied Materials Research, University of Applied Sciences Bielefeld, Germany

We performed computer simulations in order to study the binding kinetics of molecules which bind to or dissociate from a finite number of binding sites placed on a sensor surface. We modelled the process using a convection-diffusion equation together with a Robin boundary condition. We compared our results to experimental data obtained by Ritzefeld et al. [1] who studied the binding of PhoB to a functionalized surface by surface plasmon resonance studies. Furthermore, we conducted a parameter sweep which allows us to estimate the rate constants of association and dissociation.