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MI: Fachverband Mikrosonden

MI 1: Microanalysis and Microscopy of Biological Materials

MI 1.2: Hauptvortrag

Montag, 16. März 2015, 10:15–11:00, EMH 225

Electron Cryotomography of Archaea — •Bertram Daum — Max-Planck Institut für Biophysik, Frankfurt/Main, Deutschland

Electron cryotomography is an electron microscopic technique capable of visualising cellular structures in three dimensions. By combining newly developed direct electron detectors and subtomogram averaging of repetitive particles, it is possible to acquire in situ structures of proteins complexes at sub-nanometre resolution. We employ this powerful technique to investigate membrane protein complexes involved in cell-cell interactions as well as viral infection of Archaea, a group of microorganisms that next to Bacteria and Eukaryotes forms the third branch of evolution, and often populates extreme environments.