Berlin 2015 – scientific programme

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MI: Fachverband Mikrosonden

MI 1: Microanalysis and Microscopy of Biological Materials

MI 1.4: Talk

Monday, March 16, 2015, 11:15–11:30, EMH 225

Characterizing the Material Bone by Combining Microscopy Methods and X-ray Scattering — •Wolfgang Wagermaier — Max Planck Institute of Colloids and Interfaces, Department of Biomaterials, Potsdam, Germany

The structure of bone at all hierarchical levels continuously adapts during growth and healing. The local characteristics of mineral particles and the organic matrix in bone can be investigated to elucidate basic biological processes. Scanning electron microscopy is used to quantify the amount of mineral, while size and orientation of mineral particles are characterized using small and wide angle X-ray scattering. A combination of rhodamine staining and confocal laser scanning microscopy enables a three-dimensional visualization of voids in bone, housing blood vessels and bone cells. This combination of methods enables a visualization of soft and hard tissue components and to estimate their properties in relation to the spatial tissue organization.