Berlin 2015 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
O: Fachverband Oberflächenphysik
O 41: Inorganic/Organic Interfaces
O 41.5: Poster
Dienstag, 17. März 2015, 18:15–21:00, Poster B
Templated Protein Adsorption on Cu2N — •Sebastian Koslowski1, Daniel P. Rosenblatt1, Sabine Abb1, Stephan Rauschenbach1, Klaus Kern1,2, and Uta Schlickum1 — 1Max-Planck-Institut für Festkörperforschung, Heisenbergstrasse 1, D-70569 Stuttgart,Germany — 2Institut de Physique de la Matière Condensée, Ecole Polytechnique Fédérale de Lausanne (EPFL),CH-1015 Lausanne, Switzerland
In recent investigations of the protein Cytochrome-C (CytC) deposited on metallic substrates by electrospray ion beam deposition (ES-IBD) it has been shown that the adsorption conformation of the unfolded protein is strongly influenced by surface mobility and the charge state of the gas phase protein ion [1]. The length and the flexibility of the peptide chain allows for a large conformational freedom in the gas phase as well as on homogenous surfaces.
Here we present scanning tunneling microscopy (STM) investigations of the conformation of single CytC molecules deposited by ES-IBD on a Cu2N monolayer. Cu2N forms an adlayer with a square grid-like superstructure on the underlying Cu(100) substrate and acts as a template for the deposited proteins. This reduces their conformational freedom. We analyzed the surface conformations of CytC using models, which describe polymer adhesion on a grid with and without the assumption of memory of the gas phase conformation. The acquired data are best described by a model, in which elongated molecules try to reach the pure Cu rows with minimal movement.
Z. Deng et al., Nano Lett. 12 (2012) 2452-2458