Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe

Q: Fachverband Quantenoptik und Photonik

Q 62: Poster: Quantum Optics and Photonics III

Q 62.71: Poster

Donnerstag, 26. März 2015, 17:00–19:00, C/Foyer

Angstrom-Resolution Cryogenic Localization Microscopy — •Siegfried Weisenburger, Luxi Wei, and Vahid Sandoghdar — Max Planck Institute for the Science of Light and Department of Physics, Friedrich-Alexander University Erlangen-Nürnberg, 91058 Erlangen

The significance of super-resolution microscopy beyond the diffraction limit was recognized by the Nobel Prize in Chemistry in 2014. One of the super-resolution techniques is based on finding the position of single fluorophores by determining the center of their point-spread functions with arbitrary localization precision, whereby the latter depends on the available signal-to-noise ratio. We recently demonstrated Angstrom localization precision made possible by the substantial improvement of the molecular photostability at cryogenic temperatures [1]. We have now demonstrated a cryogenic colocalization microscopy method with two fluorophores on the backbone of a double-stranded DNA [2]. By measuring the separations of fluorophore pairs placed at various design positions, we verify the feasibility of cryogenic distance measurement with sub-nanometer accuracy. We discuss the challenges of our methodology and our progress towards the measurement of intramolecular distances in proteins and other biomolecules.

[1] S. Weisenburger, B. Jing, A. Renn, and V. Sandoghdar, Proc. SPIE 8815, 88150D (2013).

[2] S. Weisenburger, B. Jing, D. Hänni, L. Reymond, B. Schuler, A. Renn, and V. Sandoghdar, ChemPhysChem 15, 763 (2014).