Hannover 2016 – scientific programme

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MO: Fachverband Molekülphysik

MO 1: Quantum Control and Femtosecond Spectroscopy 1

MO 1.6: Talk

Monday, February 29, 2016, 12:15–12:30, f102

Time-resolved Circular Dichroism Spectroscopy of Hemoglobin — •Heiko Hildenbrand, Andreas Steinbacher, Federico Koch, Marco Schmid, and Tobias Brixner — Institut für Physikalische und Theoretische Chemie, Universität Würzburg, Am Hubland, 97074 Würzburg

Circular dichroism (CD) is a common technique to analyze chiral samples. However, the typically utilized long optical path lengths, high concentrations, and long integration times in the steady state are not suitable for ultrafast spectroscopy. Hence, only few chirality-sensitive spectroscopic approaches in the liquid phase are known in the literature [1].

Here, we present broadband time-resolved CD spectroscopy which is based on a setup capable of mirroring an arbitrary polarization state of an ultrashort laser pulse. Hence, by passing a broadband probe pulse through this setup we can switch between opposite handedness on a shot-to-shot basis to detect pump-induced CD changes. To demonstrate the capabilities of this approach we investigated the early photochemistry of oxygenated hemoglobin and myoglobin by time-resolved circular dichroism and transient absorption spectroscopy in the visible spectral region, since the spectrometer is also capable of acquiring both signals simultaneously.

[1] Meyer-Ilse et al., Laser Photon. Rev. 7, 495 (2013)