Hannover 2016 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
MO: Fachverband Molekülphysik
MO 9: Biomolecules and Photochemistry
MO 9.2: Vortrag
Dienstag, 1. März 2016, 15:00–15:15, f102
Multiphoton microscopy for corneal pathologies diagnosis — •Ana Batista1,2, Hans Georg Breunig1,3, Aisada Uchugonova1, Berthold Seitz4, António Miguel Morgado2,5, and Karsten König1,3 — 1Department of Biophotonics and Laser Technology, Saarbrücken, Germany — 2Institute for Biomedical Imaging and Life Sciences, Coimbra, Portugal — 3JenLab GmbH, Jena, Germany — 4Department of Ophthalmology, University of Saarland, Medical Center, Homburg/Saar, Germany — 5Department of Physics, Coimbra, Portugal
The diagnosis of corneal pathologies may be improved by multiphoton microscopy (MPM), which provides 3D imaging of the cornea with subcellular resolution, and information on tissue morphology and cells' metabolism. Here, we report on the use of MPM for ex-vivo characterization of non-pathological human corneas and evaluation of structural and metabolic changes due to pathology. A 5D multiphoton microscope with high spatial, temporal, and spectral resolutions was used for image acquisition.
All layers of the human cornea were characterized ex-vivo based on their autofluorescence and SHG. The autofluorescence lifetime of the metabolic co-factors NAD(P)H and flavins was used to assess the cells' metabolic state. Corneal samples with different pathologies showed significant differences in the metabolism of corneal epithelial cells. Different collagen fibrils structural organizations as well as autofluorescence lifetimes were observed in the corneal stroma of pathological samples. Discrimination between different pathologies was also feasible.