Hannover 2016 – scientific programme
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MS: Fachverband Massenspektrometrie
MS 6: Posters II
MS 6.2: Poster
Wednesday, March 2, 2016, 16:30–19:00, Empore Lichthof
Atmospheric picosecond IR laser desorption by impulsive vibrational excitation mass spectrometry (PIRL-DIVE-MS) — •Yinfei Lu1, Cornelius L. Pieterse1, Wesley D. Robertson1, and R. J. Dwayne Miller1,2 — 1Max Planck Institute for the Structure and Dynamics of Matter, Hamburg, Germany — 2University of Toronto, Toronto, Canada
Desorption by impulsive vibrational excitation (DIVE), using a recently developed picosecond infrared laser (PIRL), has been shown to be an efficient and ultra-soft laser ablation method for the extraction of biomolecules from tissue and solution free of thermal and acoustic damage. DIVE ablation has been shown to efficiently extract intact and even functional proteins, protein complexes and viruses. Here we apply DIVE ablation for the development of a new extraction and ionization technique for mass spectrometry. We demonstrate the efficient production of gas phase ions of small molecules, peptides and large proteins from bulk water for ultra-high sensitivity (femto-mole) mass spectrometry using DIVE in combination with a novel mass spectrometry interface. No further ionization or nebulization methods are employed. The system is directly compared to standard electrospray ionization (ESI). Further, a nanofabricated, dynamically wetted, pico-liter chip array system for aqueous DIVE-MS is demonstrated by utilizing custom image processing base fiducial registration software for sample loading and sampling. The method represents an efficient method for ultra-high sensitivity extraction and MS detection of analytes directly from lab on chip constructions.