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Q: Fachverband Quantenoptik und Photonik

Q 32: Biophotonics

Q 32.3: Vortrag

Mittwoch, 2. März 2016, 11:30–11:45, f342

Aberration correction in STED nanoscopy for super-resolution imaging deep inside living tissue — •Jasmin K. Pape, Nicolai T. Urban, Jennifer-M. Masch, and Stefan W. Hell — Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Göttingen, Germany

Stimulated emission depletion (STED) nanoscopy is a far-field fluorescence imaging technique capable of resolving structures on the nanometer scale. It is remarkably well suited for dynamic imaging of live cells and tissues, especially due to its short acquisition times. When imaging deep inside living tissue, however, two main factors limit the imaging performance: one is the loss of intensity due to absorption and scattering, the other is the distortion of the wavefront shape caused by the inhomogeneous refractive index inside the sample medium.

We address the latter problem by pre-shaping the wavefront of the STED beam using a spatial light modulator, with the aim of recovering a high-quality donut-shaped intensity distribution, which is essential for achieving high spatial resolutions. We determine the optimal correction parameters for compensating the sample-induced aberrations by employing an algorithm which records a series of images and then evaluates different properties of each image. The correction process is improved in a way that reduces the number of acquisitions necessary to find the best correction. The correction capabilities of this method will be compared both in artificial samples and in live cells.