Hannover 2016 – scientific programme

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Q: Fachverband Quantenoptik und Photonik

Q 59: Poster: Symposium Biomedical Optics (SYBO)

Q 59.3: Poster

Thursday, March 3, 2016, 16:30–19:00, Empore Lichthof

Angular resolved light scattering microscopy on human chromosomes — •Dennis Müller, Julian Stark, and Alwin Kienle — Institute for Lasertechnologies in Medicine and Metrology at Ulm University

Common optical methods to karyotype human chromosomes rely on marker substances. Scattering light microscopy promises to be a method to distinguish human chromosomes marker-free, when supported with suitable theoretical models.

The scattering light microscope setup consists of an inverse microscope with an AOTF - supercontinuum laser combination as source, providing collimated broadband light and monochromatic light with a tuneable wavelength at high intensities. The backscattered light can be sampled with a spectrometer or angular resolved via a CCD camera, positioned in the Fourier plane [1].

With this setup, the angular resolved scattering pattern of human chromosomes were measured. The measurement conditions were modelled using the Amsterdam Discrete Dipole Approximation (ADDA), an open source light scattering simulation software based on Maxwell's equations. Measurement and simulation results show a high correlation with a strong sensibility to the chromosome's geometrical properties. Hence, this method promises to become a powerful, marker-free tool in measuring geometrical features of organic samples well below the resolution limit of common light microscopy.

[1] M. Schmitz, T. Rothe, A. Kienle, Biomed. Opt. Express, Vol.2, No. 9, p.2665-2678 (2011)