Regensburg 2016 – scientific programme

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BP: Fachverband Biologische Physik

BP 11: Bioimaging and Spectroscopy II

BP 11.1: Talk

Monday, March 7, 2016, 15:00–15:15, H43

Cellular Structures Resolved by X-Ray Diffraction with Micro- and Nanometer Beamsize: From Stem Cells to Cardiomyocytes — •Marten Bernhardt¹, Jan-David Nicolas¹, Marius Priebe¹, Markus Osterhoff¹, Carina Wollnik², Ana Diaz³, Marina Eckermann¹, Florian Rehfeldt², and Tim Salditt¹ — ¹Institute for x-ray physics, Göttingen — ²Third Institute of Physics - Biophysics, Göttingen — ³Paul Scherrer Institut, Villigen

High resolution scanning small angle x-ray scattering (scanning SAXS) enables an access to local cellular structures on a mesoscopic scale. We have performed micro- and nanofocus SAXS recordings on naive human mesenchymal stem cells (hMSCs), neonatal rat cardiomyocytes and other differentiated cell lines and characterized their 2D-diffraction patterns: Results on freeze-dried samples reveal naive hMSCs to be rather weak scatterers with little anisotropic scattering behavior. In contrast, disassembled cells from neonatal rat tissue show a strong anisotropic diffraction signal, that enable us to track down filamentous structures by automated principal component analysis (PCA). These structures can be correlated to the visible light micrograph of fluorescently labeled actin. Successful wet chamber experiments provide a basis for future single cell recordings in chemically fixated and alive states.