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Regensburg 2016 – scientific programme

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BP: Fachverband Biologische Physik

BP 11: Bioimaging and Spectroscopy II

BP 11.1: Talk

Monday, March 7, 2016, 15:00–15:15, H43

Cellular Structures Resolved by X-Ray Diffraction with Micro- and Nanometer Beamsize: From Stem Cells to Cardiomyocytes — •Marten Bernhardt1, Jan-David Nicolas1, Marius Priebe1, Markus Osterhoff1, Carina Wollnik2, Ana Diaz3, Marina Eckermann1, Florian Rehfeldt2, and Tim Salditt11Institute for x-ray physics, Göttingen — 2Third Institute of Physics - Biophysics, Göttingen — 3Paul Scherrer Institut, Villigen

High resolution scanning small angle x-ray scattering (scanning SAXS) enables an access to local cellular structures on a mesoscopic scale. We have performed micro- and nanofocus SAXS recordings on naive human mesenchymal stem cells (hMSCs), neonatal rat cardiomyocytes and other differentiated cell lines and characterized their 2D-diffraction patterns: Results on freeze-dried samples reveal naive hMSCs to be rather weak scatterers with little anisotropic scattering behavior. In contrast, disassembled cells from neonatal rat tissue show a strong anisotropic diffraction signal, that enable us to track down filamentous structures by automated principal component analysis (PCA). These structures can be correlated to the visible light micrograph of fluorescently labeled actin. Successful wet chamber experiments provide a basis for future single cell recordings in chemically fixated and alive states.

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