Regensburg 2016 – scientific programme
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BP: Fachverband Biologische Physik
BP 11: Bioimaging and Spectroscopy II
BP 11.2: Talk
Monday, March 7, 2016, 15:15–15:30, H43
Imaging proteins at the truly single molecule level — •Jean-Nicolas Longchamp1, Stephan Rauschenbach2, Sabine Abb2, Conrad Escher1, Tatiana Latychevskaia1, Klaus Kern2,3, and Hans-Werner Fink1 — 1Physics Department of the University of Zurich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland — 2Max Planck Institute for Solid State Research, Heisenbergstrasse 1, DE-70569 Stuttgart, Germany — 3Institut de Physique de la Matière Condensée, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland
Imaging a single protein has been a long-standing dream for advancing structural biology and with this various fields in natural science. Here I will show that, sub-nanometer resolved images of individual folded proteins have been obtained for the first time ever. Electrospray ionization for the specific selection and sound deposition of individual proteins onto ultraclean freestanding graphene in an ultra-high vacuum environment and low-energy electron holography for the non-destructive imaging are combined in a novel experimental workflow.