Regensburg 2016 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 11: Bioimaging and Spectroscopy II
BP 11.3: Vortrag
Montag, 7. März 2016, 15:30–15:45, H43
Developing a modular sensor platform for intracellular environmental sensing — •Torsten Rendler1, Jitka Slegerova2, Ondrej Zemek3, Jan Kotek3, Andrea Zappe1, Zhiqin Chu1, Petr Cigler2, and Jörg Wrachtrup1 — 13. Physikalisches Institut, Universität Stuttgart, Deutschland — 2Institute of Organic Chemistry and Biochemistry AS CR, Flemingovo nam. 2, Prague 6, Czech Republic — 3Department of Inorganic Chemistry, Faculty of Science, Charles University, Hlavova 8, Prague 2, Czech Republic
The development of nano-scaled sensors that can be embedded intracellular without minor modification on the local environments are important for various biological and medical applications. For this purpose we utilize the nitrogen vacancy (NV) center in nanodiamond providing close to single spin sensitivity and nanometer resolution at ambient conditions, providing at the same time long term stability and low cytotoxicity in biological systems. By combining the well-established clinical magnetic resonance imaging (MRI) agent namely Gadolinium with NV spin relaxometry in nanodiamonds, we developed a hybrid sensor that can be adjusted to monitor various physiological quantizes. As an example we demonstrate sensitivity to pH and changes in the redox potential at submicron length scales in a microfluidic channel. Furthermore we introduce our hybrid sensor system into cells and investigate it's response on the local cellular environment. The current work shall open a novel approach for studying subtle changes in physiological conditions, pointing to a new pathway for diagnosis and therapeutics at subcellular level.