Regensburg 2016 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 13: Posters - Anomalous Diffusion in Complex Environments

BP 13.2: Poster

Montag, 7. März 2016, 17:30–19:30, Poster C

Subcellular Organization of Eukaryotes during Mitosis — Nisha Pawar, •Claudia Donth, and Matthias Weiss — Universität Bayreuth, Experimentalphysik 1

Eukaryotic cells undergo major structural changes at the onset and during mitosis. This includes nuclear envelope breakdown, the formation of a mitotic spindle, disassembly of the Golgi apparatus and a transition of the endoplasmic reticulum from netlike to sheetlike structure.

Using spatially resolved FCS measurements we were able to show that the contiguous fluid of former cytoplasm and nucleoplasm features an anisotropically varying diffusion characteristics in the mitotic spindle area [1]. This affects the preferential direction of long-time diffusion as well as the distribution of nucleocytoplasmic constituents throughout the spindle region.

While being functionally closely related in interphase, ER and Golgi apparatus were found to be inherited separately: After its disassembly, Golgi components are spread throughout the whole cell, while the ER takes on a sheetlike structure that is excluded from the mitotic spindle region. Starting from an unmixed state of ER and Golgi components, Golgi reassembly after cytokinesis appears to be independent of the ER.

[1] N. Pawar, C. Donth and M. Weiss; Curr. Biol. 24, 16, 1905-1908 (2014)