Regensburg 2016 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 18: Posters - DNA, RNA and Related Enzymes
BP 18.3: Poster
Montag, 7. März 2016, 17:30–19:30, Poster C
The maximum number of independently hybridizing DNA strands — •Mina Mohammadi-Kambs1, Kathrin Hölz2, Mark Somoza2, and Albrecht Ott1 — 1Universität des Saarlandes, Department of Experimental Physics — 2University of Vienna, Institute of Inorganic Chemistry, Faculty of Chemistry
In the cell molecular information processing is based on molecular recognition and binding. Although DNA hybridization is sometimes understood as lock and key interaction, it is not completely clear how the two molecules can identify each other. Even with a few mismatched bases, hybridization still occurs and this makes it difficult to predict hybridization in crowded and competitive environments. Here we study how different strands need to be to avoid competition for the same molecule. In this work we first numerically derive the maximal number of possible sequences, which can coexist without competing to bind to each other's perfect match. Experimentally we determine the appropriate minimum number of mismatched bases and investigate the behavior of DNA in a scenario where many sequences bind to their surface bound complements so that competition is minimized.