Regensburg 2016 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 18: Posters - DNA, RNA and Related Enzymes

BP 18.5: Poster

Montag, 7. März 2016, 17:30–19:30, Poster C

Measuring DNA translocation forces through MoS2 nanopores — •Dennis Kreft, Sebastian Knust, and Dario Anselmetti — Bielefeld University

We measured the forces acting on a single strand of dsDNA during translocation through nanopores in molybdenum disulfide (MoS2) mono- and bilayer membranes by Optical Tweezers. The system includes a video-based force detection and analysis system allowing for virtually interference-free axial force measurements [1].

Preliminary measurements of the translocation of a λ-DNA dimer through a 40 nm Helium-ion drilled nanopore in a MoS2 bilayer resulted in a force of (4.5±1.5) pN @ 50 mV. We will show further measurements performed with an overall force resolution of 0.5 pN at a sample rate of 2042 Hz.

[1] S. Knust et. al., Rev. Sci. Instrum. 83, 103704 (2012)