Regensburg 2016 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 19: Posters - Membranes and Vesicles
BP 19.11: Poster
Montag, 7. März 2016, 17:30–19:30, Poster C
X-ray reflectivity investgation of structure and kinetics of photoswitchable lipid monolayers — •Kuntal Chatterjee1, Björn Haushahn1, Chen Shen1,3, Sven Festersen1, Jonas Warias1, Benjamin Runge1, Franziska Reise4, Thisbe Lindhorst4, Beate Klösgen3, Olaf Magnussen1,2, and Bridget Murphy1,2 — 1Institute for Experimental and Applied Physics, University of Kiel, 24098 Kiel, Germany — 2Ruprecht Heansel Laboratory, University of Kiel, 24098 Kiel, Germany — 3University of Southern Denmark, 5230 Odense M, Denmark — 4Otto Diels Institute of Organic Chemistry, University of Kiel, 24118 Kiel, Germany
The mechanical and dynamic properties of phospholipid membranes are of importance for important biological functions, such as switching of embedded proteins. In order to investigate these properties we study model systems in which amphiphilic photoswitchable molecules are integrated into Langmuir films of phospholipids. we have modified glycolipids to contain an azobenzene photoswitch between the chain and the head group and successfully embedded those in a monolayer of dipalmitoylphosphatidylcholine (DPPC). This allows us to reversibly change the azobenzene-glycolipid orientation between trans- and cis-conformation by illumination with UV and blue light. We have followed the structural changes in this model membrane and the switching kinetics of the system with Langmuir isotherms and in situ X-ray reflectivity at the LISA diffractometer P08, PETRA III. This work is funded by SFB 677. The LISA instrument at PETRA III is funded by BMBF 05K13FK2.