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Regensburg 2016 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 19: Posters - Membranes and Vesicles

BP 19.6: Poster

Montag, 7. März 2016, 17:30–19:30, Poster C

Hydrogen bond balance and entropy determine the interaction between glycolipid membranes in plant thylakoidsMatej Kanduč1,2, •Alexander Schlaich1, Alex de Vries3, Bruno Demé4, Roland R. Netz1, and Emanuel Schneck51Fachbereich Physik, Freie Universiät Berlin, 14195 Berlin, Germany — 2Soft Matter and Functional Materials, Helmholtz-Zentrum Berlin, 14109 Berlin, Germany — 3Groningen Biomolecular Sciences and Biotechnology (GBB) Institute and Zernike Institute for Advanced Materials, University of Groningen, 9747 AG Groningen, The Netherlands — 4Institut Laue-Langevin, Grenoble, France — 5Department of Biomaterials, Max Planck Institute of Colloids and Interfaces, 14476 Potsdam, Germany

Naturally stacked biological membranes contain high amounts of glycolipids. Swelling experiments with membranes composed of glycolipids from plant thylakoids revealed that the interaction between these membranes is repulsive only at very low hydration. Even in excess water the membranes stay in close contact, which is in contrast to commonly studied phospholipid membranes.

Using solvent-explicit Molecular Dynamics simulations and taking the chemical potential of water into account, we reproduce the experimentally obtained pressure-distance curves of membranes composed of the plant glycolipid DGDG. Our analysis identifies the hydrogen bond balance and entropic contributions as the key determinants of the interaction. Furthermore, we find that even at the swelling limit the opposing membrane surfaces interact directly via hydrogen bonds.

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