Regensburg 2016 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
BP: Fachverband Biologische Physik
BP 22: Posters - Neurosciences
BP 22.4: Poster
Montag, 7. März 2016, 17:30–19:30, Poster C
Tailored Multielectrode Array as an Interface for Neuronal Networks — •Norman Shepheard1,2, Matthias Schürmann3, Ulrich Rückert2, Barabara Kaltschmidt3,4, Christian Kaltschmidt3, and Andy Thomas1,5 — 1Center for Spinelectronic Materials and Devices, Physics Department, Bielefeld University, Germany — 2Cognitronics and Sensor Systems, Bielefeld University, Germany — 3Cell Biology, Bielefeld University, Germany — 4Molecular Neurobiology, Bielefeld University, Germany — 5Leibniz Institute for Solid State and Materials Research Dresden (IFW Dresden), Institute for Metallic Materials, Dresden, Germany
To study neuronal network functions one need the appropriate interface to read out the action potential and to stimulate the neurons. We demonstrate a process to grow guided neuron networks in vitro, as well as to build multielectrode arrays (MEAs), which provides an electrode arrangement fitting to the desired network layout. Soma localization to one electrode is a key point for measurements in networks.
The fabrication of MEAs was done with UV-laser lithography and sputtered thin layers of titanium, titanium nitride for electrodes and silicon nitride as an insulator. The long time stable adhesion layer system is made of (3-aminopropyl)triethoxysilane, glutaraldehyde and poly lysine on top. The patterning of the adhesion layer system for guided neuronal networks is made via UV-laser lithography as well.
This flexible approach allows cell body localization of the neurons and neurite guidance as shown in the results. The network designs fits to the self-built MEAs.